Immunofluorescent Studies of Human Articular Cartilage

Studies on cathepsin B in human articular cartilage.

The thiol proteinase cathepsin B (EC 3.4.22.1), previously called cathepsin B1, was assayed in human articular cartilage by its hydrolysis of the synthetic substrate alpha-N-benzoyl-DL-arginine 2-naphthylamide. The enzyme was activated by cysteine and EDTA and completely inhibited by iodoacetamide and HgCl2. It was also partially inhibited by whole human serum. Human osteoarthrotic cartilage ha...

Immunofluorescent Studies in Human Leukemia1

High-speed (80,000 X g) pellets of plasma from leukemic patients and from normal individuals were used to prepare antisera in rabbits. The leukemic plasma pellets contained virus-like particles as determined by electron microscopy, whereas similar particles were not observed in the normal pellets. Globulin fractions from six antisera to normal pellets (NPA) and six antisera to leukemic pellets ...

Differential Immunohistochemical Expression Pattern of Galectin-3 in Normal and Osteoarthritic Human Articular Cartilage

Background: Previous studies have shown that Galectin-3, a member of lectin family, is expressed in developing cartilage in mouse embryo and also in growth plate of long bones.   Objective: In the present work, the expression pattern of Galectin-3 in normal and various grades of osteoarthritic (OA) human articular cartilage has been studied.   Methods: Using immunohistochemistry and standard we...

Electrokinetic evaluation of human articular cartilage

The reactions of injured human articular cartilage.

The physical and chemical structure of normal hyaline articular cartilage was described by Collins (1949), but there is little published on its reactions to injury and repair in the human subject, though animal observations have been made, notably by Bennett, Bauer and Maddock (1932). The slow metabolism and physiological inactivity of joint cartilage were emphasised by Bywaters (1937), again m...